LTC combines the rapid workflow and low input mass advantages of amplicon enrichment with the UMI integration and scalability of hybridization capture.
Simple workflow: Target capture can be completed in less than 4 hours, with only 1 hour of hands-on time, and is easily automated
Scalable: Single-tube panels have been demonstrated from <50bp to >1Mb, with exome-sized panels possible
Efficient: High uniformity and on-target regardless of panel size, leading to reduced sequencing costs
Low level variant detection: SNVs, indels, CNVs and translations are detected using duplex UMIs introduced during library prep, providing detailed molecular information including start/stop coordinates and enabling detection of unknown fusion pairs
Broad applications: include liquid biopsy and minimal residual disease, immune sequencing, metagenomics, single cell sequencing and gene-editing characterization
